Fig. S2 Localisation of cervical sympathetic and enteric neurons in 3 dpf zebrafish embryos. (A-D) Whole-mount in situ hybridisation for phox2b as marker for autonomic cells was carried out. After dissection of dorsal hindbrain/spinal cord and yolk sac, a stack of pictures was made focussing from dorsal (A) to ventral (D) through the embryo. This illustrates the dorsal location of the cervical sympathetic ganglion (dashed circle), restricted to a relatively short anteroposterior area (A). Enteric neurons (white arrowheads) that appear as diffuse staining in A show up in more ventral focal planes (B-D), and extend to more rostral and caudal regions down to the anus (not shown). (E) An optical overlay of images in A to D to illustrate the different lateral positions of enteric and cervical sympathetic ganglion cells.
Image
Figure Caption
Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Development