Fig. 8 The medial pharyngeal endoderm is not affected by retinoic acid. A: Ventral view of foxa2 in situ hybridization of 30-hpf control embryo. (B) Embryo treated with DEAB between 4-10 hpf and (C) embryo treated with DEAB between 16-30 hpf. We did not find any significant difference in the length of the expression domain in control versa treated embryos, based on the ratios between the length of the pharyngeal endoderm (distance "a") and the length of the brain (diencephalon) plus the pharyngeal endoderm (distance "b"). D: Statistical analysis of ratios "a"/"b" in embryos treated with DEAB between 4-10 hpf during gastrulation, treated between 16-30 hpf post-gastrulation and untreated control embryos.
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