ZFIN Image: Raeker et al., 2006, Fig. 2

Image

Figure Caption

Fig. 2 Expression of obscurin and obscurin-MLCK in developing zebrafish embryos. A: RT-PCR of total RNA from zebrafish embryos using primers from the 5' end of the obscurin/obscurin-MLCK gene. RNA samples were prepared from whole embryos 15, 22, 24, 36, 48, 60, and 72 hr post-fertilization (hpf) and from adult zebrafish hearts. Expression is first noted in embryos approximately 22 hpf with expression persisting in the adult heart. B: Immunolocalization of obscurin/obscurin-MLCK expression using the link7 and Ank antibodies in zebrafish embryos at 48 hpf. Note the expression of obscurin and obscurin-MLCK in the heart (∧) and skeletal muscle (arrows and inset, far right). Immunolocalization of myosin (MF20 antibody), which decorates the A bands of striated muscle, is shown for comparison. Note that, unlike myosin, both obscurin and obscurin-MLCK are also expressed in non-muscle tissues, including the central nervous system (*), at this stage in development.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.