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Fig. 2 msn1 encodes a zebrafish orthologue of Drosophila Misshapen and is expressed during gastrulation. (A) Phylogenetic analysis of zebrafish homologues (Msn1-3) of Drosophila Misshapen. The scale bar indicates point mutations per site. Msn1 is most closely related to human and mouse Traf2_NCK (Traf2 and NCK interacting kinase). (B) Protein domain comparison of zebrafish Msn1 and Drosophila Misshapen. Percentage amino acid identity between conserved N-terminal Ser/Thr kinase and C-terminal CNH (citron homology) domains is indicated. (C-E) msn1 mRNA expression during early cell division gastrulation stages. In situ hybridization of wild-type embryos at 64-cell (C), shield (D) and bud stages (E). Dorsal is to the right in D and E. Scale bar in E: 100 μm for C-E.