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Figure Caption
Fig. 5 Specificity of the anti-Zf-T antiserum. Coomassie-blue stained SDS-PAGE gels (lanes A and B) and Western blot (lanes C and D) showing total protein from induced bacteria carrying expression vector pET-ZfT (lanes A and C) and from uninduced bacteria also containing the expression vector (lanes B and D). The band with apparent relative molecular mass of 45x103 was used to raise the antiserum. Immunprecipitations using immune (lane E) and preimmune serum (lane F) to precipitate in vitro made Zf-T protein further demonstrates specificity of the antiserum.
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