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Fig. 4 Regulation of lhx2 expression. (A,B) Wild-type embryos were treated with SU5402 (B) or DMSO carrier (A) from 6 hpf to 10 hpf and labeled to show lhx2 expression. Blocking Fgf signaling during the onset of lhx2 expression nearly eliminated lhx2 expression in the embryo (B, arrowhead). Insets show complete loss of the Fgf-regulated gene erm in the forebrain and hindbrain of identically treated embryos (arrows). (C,D) Treating embryos with SU5402 from 10 hpf to 24 hpf reduced lhx2 expression in the telencephalon (arrowheads) and diencephalon (arrow). All panels show lateral views of the head, anterior to the left. Eyes were removed in C and D.