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Fig. 5 Antisense morpholino and dominant-negative construct of Pcdh10 disturbed somitogenesis. b-f: Embryos were injected with pcdh10 antisense morpholino oligonucleotide (MO), pcdh10mo, fixed at 14-15 hours postfertilization (hpf), and stained by in situ hybridization (ISH) for MyoD to show somite phenotypes. Somitogenesis was scored as affected when (AS) left-right asymmetry was found in some somites (arrowheads in b); (BR) there were bridges (right arrowhead in c), compression of adjacent somites (arrowhead in d) or blurred somite boundaries (left arrowhead in c); or (MS) some somites were missing or misplaced (arrowheads in e), or the midline structures were widened and disorganized (f). a: Embryo injected with negative control MO with normal morphologies. g,h: Embryos were injected with Pcdh10ecF RNA at the four-cell stage. Embryos with unilateral expression of GFP as a tracking dye (arrowheads in g) were fixed for MyoD ISH. Note the deformation of somites (arrowheads in h) ipsilateral to GFP expression. Dorsal views with rostral to the top. Scale bars = 100 μm.