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Fig. 7 Onset of sox10 expression in the terminal nerve and midbrain at 48 hpf. (A) Ventral view showing sox10 expression in the terminal nerve (blue, arrows). (B) Higher magnification view of the terminal nerve on one side, showing gnrh3-positive cells (arrowhead, red), sox10-positive cells (arrow, blue), and cells apparently labeled for sox10 and gnrh3 (arrow with asterisk). (C) Lateral view showing sox10 (blue, arrow) and gnrh2 (red, arrowhead) expression in the midbrain. (D) Higher magnification view of the gnrh2-positive cells (arrowhead, red) and sox10-positive cells (arrow, blue); there is no co-localization of sox10 and gnrh2 at this stage in development. Scale bars: A,C, 50 µm; B,D, 40 µm.