Gene
mgat5
- ID
- ZDB-GENE-060616-238
- Name
- alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase
- Symbol
- mgat5 Nomenclature History
- Previous Names
-
- mgat5a (1)
- si:dkeyp-4h4.2
- zgc:136939
- Type
- protein_coding_gene
- Location
- Chr: 22 Mapping Details/Browsers
- Description
- Predicted to enable alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity. Acts upstream of or within neuromast hair cell differentiation involved in neuromast regeneration. Predicted to be located in Golgi membrane and extracellular region. Predicted to be active in Golgi apparatus. Is expressed in gut and nervous system. Orthologous to human MGAT5 (alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 1 figure from Pei et al., 2016
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
- No data available
Wild Type Expression Summary
- All Phenotype Data
- No data available
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| hg29 | Allele with one deletion | Unknown | Unknown | CRISPR | |
| la022146Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| mn0157Gt | Transgenic insertion | Unknown | Unknown | DNA | |
| sa10529 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa13731 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa17972 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| sa24101 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| sa43790 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| sa45756 | Allele with one point mutation | Unknown | Splice Site | ENU |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-mgat5 | Varshney et al., 2015 | |
| CRISPR2-mgat5 | (2) | |
| CRISPR3-mgat5 | (4) | |
| CRISPR4-mgat5 | (5) |
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Human Disease
Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Additional Resources | Length | Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase | Glycosyltransferase family 18, catalytic domain | MGT5A-like, N-terminal |
|---|---|---|---|---|---|
| UniProtKB:Q1ECU6 | InterPro | 742 |
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Interactions and Pathways
No data available
Plasmids
No data available
No data available
| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH73-124B14 | ZFIN Curated Data | |
| Contained in | BAC | CH73-125N14 | ZFIN Curated Data | |
| Contained in | BAC | DKEYP-4H4 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:136939 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_001045311 (1) | 5439 nt | ||
| Genomic | GenBank:CU571316 (1) | 125605 nt | ||
| Polypeptide | UniProtKB:Q1ECU6 (1) | 742 aa |
- Matheny-Rabun, C., Mokashi, S.S., Radenkovic, S., Wiggins, K., Dukes-Rimsky, L., Angel, P., Ghesquiere, B., Kozicz, T., Steet, R., Morava, E., Flanagan-Steet, H. (2024) O-GlcNAcylation modulates expression and abundance of N-glycosylation machinery in an inherited glycosylation disorder. Cell Reports. 43:114976114976
- Ichino, N., Serres, M.R., Urban, R.M., Urban, M.D., Treichel, A.J., Schaefbauer, K.J., Greif, L.E., Varshney, G.K., Skuster, K.J., McNulty, M.S., Daby, C.L., Wang, Y., Liao, H.K., El-Rass, S., Ding, Y., Liu, W., Anderson, J.L., Wishman, M.D., Sabharwal, A., Schimmenti, L.A., Sivasubbu, S., Balciunas, D., Hammerschmidt, M., Farber, S.A., Wen, X.Y., Xu, X., McGrail, M., Essner, J.J., Burgess, S.M., Clark, K.J., Ekker, S.C. (2020) Building the vertebrate codex using the gene breaking protein trap library. eLIFE. 9:
- Pei, W., Xu, L., Huang, S.C., Pettie, K., Idol, J., Rissone, A., Jimenez, E., Sinclair, J.W., Slevin, C., Varshney, G.K., Jones, M., Carrington, B., Bishop, K., Huang, H., Sood, R., Lin, S., Burgess, S.M. (2018) Guided genetic screen to identify genes essential in the regeneration of hair cells and other tissues. NPJ Regenerative medicine. 3:11
- Pei, W., Huang, S.C., Xu, L., Pettie, K., Ceci, M.L., Sánchez, M., Allende, M.L., Burgess, S.M. (2016) Loss of Mgat5a-mediated N-glycosylation stimulates regeneration in zebrafish.. Cell regeneration (London, England). 5:3
- Carrington, B., Varshney, G.K., Burgess, S.M., Sood, R. (2015) CRISPR-STAT: an easy and reliable PCR-based method to evaluate target-specific sgRNA activity. Nucleic acids research. 43(22):e157
- Elkon, R., Milon, B., Morrison, L., Shah, M., Vijayakumar, S., Racherla, M., Leitch, C.C., Silipino, L., Hadi, S., Weiss-Gayet, M., Barras, E., Schmid, C.D., Ait-Lounis, A., Barnes, A., Song, Y., Eisenman, D.J., Eliyahu, E., Frolenkov, G.I., Strome, S.E., Durand, B., Zaghloul, N.A., Jones, S.M., Reith, W., Hertzano, R. (2015) RFX transcription factors are essential for hearing in mice. Nature communications. 6:8549
- Varshney, G.K., Pei, W., LaFave, M.C., Idol, J., Xu, L., Gallardo, V., Carrington, B., Bishop, K., Jones, M., Li, M., Harper, U., Huang, S.C., Prakash, A., Chen, W., Sood, R., Ledin, J., Burgess, S.M. (2015) High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9. Genome research. 25(7):1030-42
- Varshney, G.K., Lu, J., Gildea, D., Huang, H., Pei, W., Yang, Z., Huang, S.C., Schoenfeld, D.S., Pho, N., Casero, D., Hirase, T., Mosbrook-Davis, D.M., Zhang, S., Jao, L.E., Zhang, B., Woods, I.G., Zimmerman, S., Schier, A.F., Wolfsberg, T., Pellegrini, M., Burgess, S.M., and Lin, S. (2013) A large-scale zebrafish gene knockout resource for the genome-wide study of gene function. Genome research. 23(4):727-735
- Clark, K.J., Balciunas, D., Pogoda, H.M., Ding, Y., Westcot, S.E., Bedell, V.M., Greenwood, T.M., Urban, M.D., Skuster, K.J., Petzold, A.M., Ni, J., Nielsen, A.L., Patowary, A., Scaria, V., Sivasubbu, S., Xu, X., Hammerschmidt, M., and Ekker, S.C. (2011) In vivo protein trapping produces a functional expression codex of the vertebrate proteome. Nature Methods. 8(6):506-512
- Wang, D., Jao, L.E., Zheng, N., Dolan, K., Ivey, J., Zonies, S., Wu, X., Wu, K., Yang, H., Meng, Q., Zhu, Z., Zhang, B., Lin, S., and Burgess, S.M. (2007) Efficient genome-wide mutagenesis of zebrafish genes by retroviral insertions. Proceedings of the National Academy of Sciences of the United States of America. 104(30):12428-12433
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