Gene
irx1a
- ID
- ZDB-GENE-040707-1
- Name
- iroquois homeobox 1a
- Symbol
- irx1a Nomenclature History
- Previous Names
-
- ziro1a (1)
- zgc:109702
- zgc:136490
- Type
- protein_coding_gene
- Location
- Chr: 16 Mapping Details/Browsers
- Description
- Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within several processes, including central nervous system neuron differentiation; embryonic morphogenesis; and eye development. Predicted to be active in nucleus. Is expressed in several structures, including liver; nervous system; neural plate; pericardial region; and pleuroperitoneal region. Human ortholog(s) of this gene implicated in congenital heart disease. Orthologous to human IRX1 (iroquois homeobox 1).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 25 figures from 16 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
- No data available
Wild Type Expression Summary
- All Phenotype Data
- 11 figures from 4 publications
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| el830 | Allele with one delins | Exon 1 | Unknown | CRISPR | |
| sa15451 | Allele with one point mutation | Unknown | Missense, Splice Site | ENU | |
| sa24999 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa42638 | Allele with one point mutation | Unknown | Missense, Premature Stop | ENU | |
| sa45558 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| zf3177 | Allele with one insertion | Unknown | Frameshift | TALEN |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-irx1a | Farmer et al., 2021 | |
| MO1-irx1a | N/A | (2) |
| MO2-irx1a | N/A | (2) |
| MO3-irx1a | N/A | (3) |
| MO4-irx1a | N/A | Choy et al., 2010 |
| TALEN1-irx1a | Jung et al., 2019 |
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Human Disease
Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Additional Resources | Length | Homedomain-like superfamily | Homeobox, conserved site | Homeodomain | Iroquois-class homeodomain protein | KN homeodomain |
|---|---|---|---|---|---|---|---|
| UniProtKB:Q8JH33 | InterPro | 419 | |||||
| UniProtKB:Q90XQ7 | InterPro | 426 |
Interactions and Pathways
No data available
Plasmids
No data available
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| Tg1(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-irx1a-LOXP-gata2a:EGFP-5HS4) |
|
| 3 | (4) | |
| Tg2(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-irx1a-LOXP-gata2a:EGFP-5HS4) |
|
| 3 | (5) |
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| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH73-211K12 | ZFIN Curated Data | |
| Contains | SSLP | z9535 | Dildrop et al., 2004 | |
| Encodes | cDNA | MGC:109702 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:136490 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:195323 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_207184 (1) | 1780 nt | ||
| Genomic | GenBank:CU855690 (2) | 67458 nt | ||
| Polypeptide | UniProtKB:Q90XQ7 (1) | 426 aa |
- England, S.J., Rusnock, A.K., Mujcic, A., Kowalchuk, A., de Jager, S., Hilinski, W.C., Juárez-Morales, J.L., Smith, M.E., Grieb, G., Banerjee, S., Lewis, K.E. (2023) Molecular analyses of zebrafish V0v spinal interneurons and identification of transcriptional regulators downstream of Evx1 and Evx2 in these cells. Neural Development. 18:88
- Wesselman, H.M., Gatz, A.E., Pfaff, M.R., Arceri, L., Wingert, R.A. (2023) Estrogen Signaling Influences Nephron Segmentation of the Zebrafish Embryonic Kidney. Cells. 12(4):
- Halabi, R., Cechmanek, P.B., Hehr, C.L., McFarlane, S. (2022) Semaphorin3f as a cardiomyocyte derived regulator of heart chamber development. Cell communication and signaling : CCS. 20:126
- Hehr, C.L., Halabi, R., McFarlane, S. (2022) Spatial regulation of amacrine cell genesis by Semaphorin 3f. Developmental Biology. 491:66-81
- Weaver, N.E., Healy, A., Wingert, R.A. (2022) gldc Is Essential for Renal Progenitor Patterning during Kidney Development. Biomedicines. 10(12):
- Farmer, D.T., Patel, P., Choi, R., Liu, C.Y., Crump, J.G. (2021) Comprehensive series of Irx cluster mutants reveals diverse roles in facial cartilage development. Development (Cambridge, England). 148(16)
- Chambers, B.E., Gerlach, G.F., Clark, E.G., Chen, K.H., Levesque, A.E., Leshchiner, I., Goessling, W., Wingert, R.A. (2019) Tfap2a is a novel gatekeeper of nephron differentiation during kidney development. Development (Cambridge, England). 146(13):
- Jung, I.H., Jung, D.E., Chung, Y.Y., Kim, K.S., Park, S.W. (2019) Iroquois Homeobox 1 Acts as a True Tumor Suppressor in Multiple Organs by Regulating Cell Cycle Progression. Neoplasia (New York, N.Y.). 21:1003-1014
- Falisse, E., Ducos, B., Stockwell, P.A., Morison, I.M., Chatterjee, A., Silvestre, F. (2018) DNA methylation and gene expression alterations in zebrafish early-life stages exposed to the antibacterial agent triclosan. Environmental pollution (Barking, Essex : 1987). 243:1867-1877
- Morales, E.E., Handa, N., Drummond, B.E., Chambers, J.M., Marra, A.N., Addiego, A., Wingert, R.A. (2018) Homeogene emx1 is required for nephron distal segment development in zebrafish. Scientific Reports. 8:18038
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