Gene
akap12b
- ID
- ZDB-GENE-030131-9753
- Name
- A kinase (PRKA) anchor protein 12b
- Symbol
- akap12b Nomenclature History
- Previous Names
- Type
- protein_coding_gene
- Location
- Chr: 20 Mapping Details/Browsers
- Description
- Predicted to enable calmodulin binding activity and protein kinase A binding activity. Acts upstream of or within several processes, including Kupffer's vesicle development; bleb assembly; and negative regulation of Rho protein signal transduction. Located in perinuclear region of cytoplasm. Is expressed in several structures, including cardiovascular system; germ ring; mesoderm; nervous system; and tail bud. Human ortholog(s) of this gene implicated in chronic kidney disease; colorectal carcinoma; and juvenile myelomonocytic leukemia. Orthologous to human AKAP12 (A-kinase anchoring protein 12).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 14 figures from 6 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
-
- cb27 (7 images)
Wild Type Expression Summary
- All Phenotype Data
- 12 figures from 4 publications
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| ihb67 | Allele with one delins | Unknown | Unknown | CRISPR | |
| ihb68 | Allele with one deletion | Unknown | Unknown | CRISPR | |
| ihb69 | Allele with one deletion | Unknown | Unknown | CRISPR | |
| la010281Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| sa72 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa15047 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa43450 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa43451 | Allele with one point mutation | Unknown | Premature Stop | ENU |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-akap12b | (2) | |
| MO1-akap12b | N/A | (4) |
| MO2-akap12b | N/A | (5) |
| MO3-akap12b | N/A | Weiser et al., 2007 |
| MO4-akap12b | N/A | (3) |
| MO5-akap12b | N/A | (3) |
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Human Disease
Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Additional Resources | Length | A kinase-anchoring protein AKAP5 and AKAP12, calmodulin (CaM)-binding motif | A-kinase anchor protein 12 |
|---|---|---|---|---|
| UniProtKB:A0A0R4IC31 | InterPro | 1596 | ||
| UniProtKB:F2Z4T4 | InterPro | 1533 |
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| Type | Name | Annotation Method | Has Havana Data | Length (nt) | Analysis |
|---|---|---|---|---|---|
| mRNA |
akap12b-201
(1)
|
Ensembl | 648 nt | ||
| mRNA |
akap12b-202
(1)
|
Ensembl | 6,450 nt | ||
| mRNA |
akap12b-203
(1)
|
Ensembl | 7,749 nt | ||
| mRNA |
akap12b-204
(1)
|
Ensembl | 9,480 nt | ||
| mRNA |
akap12b-205
(1)
|
Ensembl | 7,621 nt |
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Interactions and Pathways
No data available
Plasmids
No data available
No data available
| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH73-109I14 | ZFIN Curated Data | |
| Contained in | BAC | CH73-192G21 | ZFIN Curated Data | |
| Contained in | BAC | DKEY-147L14 | ZFIN Curated Data | |
| Contains | SNP | rs3729026 | ZFIN Curated Data | |
| Contains | STS | chunp30253 | ||
| Encodes | EST | cb27 | Thisse et al., 2001 | |
| Encodes | EST | fb72g12 | ZFIN Curated Data | |
| Encodes | EST | fc21c03 | ||
| Encodes | EST | ibd1202 | ||
| Encodes | EST | wz11325 |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_001281788 (1) | 6521 nt | ||
| Genomic | GenBank:CR926887 (1) | 184926 nt | ||
| Polypeptide | UniProtKB:A0A0R4IC31 (1) | 1596 aa |
- Kim, J.G., Kim, H.H., Bae, S.J. (2019) Akap12beta supports asymmetric heart development via modulating the Kupffer's vesicle formation in zebrafish. BMB reports. 52(8):525-530
- Bayés, À., Collins, M.O., Reig-Viader, R., Gou, G., Goulding, D., Izquierdo, A., Choudhary, J.S., Emes, R.D., Grant, S.G. (2017) Evolution of complexity in the zebrafish synapse proteome. Nature communications. 8:14613
- Sanchez, N.E., Harty, B.L., O'Reilly-Pol, T., Ackerman, S.D., Herbert, A.L., Holmgren, M., Johnson, S.L., Gray, R.S., Monk, K.R. (2017) Whole Genome Sequencing-Based Mapping and Candidate Identification of Mutations from Fixed Zebrafish Tissue. G3 (Bethesda). 7(10):3415-3425
- Stapel, L.C., Lombardot, B., Broaddus, C., Kainmueller, D., Jug, F., Myers, E.W., Vastenhouw, N.L. (2016) Automated detection and quantification of single RNAs at cellular resolution in zebrafish embryos. Development (Cambridge, England). 143(3):540-6
- Kim, H., Kim, J., Jeong, J., Han, S., Kim, K. (2014) Akap12 is essential for the morphogenesis of muscles involved in zebrafish locomotion. Differentiation; research in biological diversity. 88(4-5):106-16
- Varshney, G.K., Lu, J., Gildea, D., Huang, H., Pei, W., Yang, Z., Huang, S.C., Schoenfeld, D.S., Pho, N., Casero, D., Hirase, T., Mosbrook-Davis, D.M., Zhang, S., Jao, L.E., Zhang, B., Woods, I.G., Zimmerman, S., Schier, A.F., Wolfsberg, T., Pellegrini, M., Burgess, S.M., and Lin, S. (2013) A large-scale zebrafish gene knockout resource for the genome-wide study of gene function. Genome research. 23(4):727-735
- Kwon, H.B., Choi, Y.K., Lim, J.J., Kwon, S.H., Her, S., Kim, H.J., Lim, K.J., Ahn, J.C., Kim, Y.M., Bae, M.K., Park, J.A., Jeong, C.H., Mochizuki, N., and Kim, K.W. (2012) AKAP12 regulates vascular integrity in zebrafish. Experimental & molecular medicine. 44(3):225-235
- Lemeer, S., Jopling, C., Gouw, J.W., Mohammed, S., Heck, A.J., Slijper, M., and den Hertog, J. (2008) Comparative phosphoproteomics of zebrafish Fyn/Yes morpholino knockdown embryos. Molecular & cellular proteomics : MCP. 7(11):2176-2187
- Weiser, D.C., Julien, K.R., Lang, J.S., and Kimelman, D. (2008) Cell shape regulation by Gravin requires N-terminal membrane effector domains. Biochemical and Biophysical Research Communications. 375(4):512-516
- Wang, D., Jao, L.E., Zheng, N., Dolan, K., Ivey, J., Zonies, S., Wu, X., Wu, K., Yang, H., Meng, Q., Zhu, Z., Zhang, B., Lin, S., and Burgess, S.M. (2007) Efficient genome-wide mutagenesis of zebrafish genes by retroviral insertions. Proceedings of the National Academy of Sciences of the United States of America. 104(30):12428-12433
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