FIGURE

Figure 5.

ID

ZDB-FIG-260723-49

Publication

Liu et al., 2026 - TMEM187 is a novel modulator in the regulation of erythropoiesis

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Figure 5.

Interference with TMEM187 expression in human primary CD34⁺ HSCs enhances erythroid differentiation. (A) Workflow with CD34⁺ HSCs. CD34⁺ HSCs were isolated from peripheral blood mononuclear cells (PBMCs), transfected every other day for 4 times, with either control (vector) or shTMEM187 plasmid during the flow frame, and followed by induction with erythropoietin (EPO, 1 U/mL). Cells were collected daily for analysis. (B-C) TMEM187 expression (top) and color change of the cell pellets (bottom) (B), and hemoglobin expression, detected by western blotting, in the induction course (C). (D) TMEM187 knockdown in CD34⁺ HSCs by shRNA. (E-G) Effects of TMEM187 on expression of FECH, HBA, and HBB (E) and iron metabolism-related genes (F) in the differentiation course of CD34⁺ HSCs, detected by western blotting, and quantified (G). dpi, days post induction. Ponceau S stains were used as internal controls in each figure. t test was used for significance. ∗P < .05; ∗∗P < .01. (H) Effects of TMEM187 on hemoglobin, detected by o-dianisidine staining in CD34⁺ cells after 2-day induction by EPO addition.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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