Fig. 3

Transient exposure of zebrafish larvae to BPF caused system-wide perturbations in adult lipids that were specific to sex and tissue. (A) Box plots show relative levels of acylcarnitine 16:0 (CAR 16:0), acylcarnitine 18:1 (CAR 18:1), and acylcarnitine 18:2 (CAR 18:2). (B) Heat map shows fold change (treated/control) for selected lipid species across organs for male and female fish. The experiment compared 12 control animals (7 males, 5 females) to 11 treated animals (5 males, 6 females). Lipids that decrease as a result of BPF exposure are shown with negative log2FC values and lipids that increase following BPF exposure with positive log2FC values. For clarity, lipids having a p-value greater than 0.05 or a fold change between 0.67 and 1.5 are displayed without color on the heatmap. Lipids with a q-value <0.20 are indicated by an asterisk. P-values were calculated by using a Mann Whitney U-test (unpaired). Q-values were calculated by using the Benjamini-Hochberg Procedure to control for false discovery rate. A more detailed heatmap including all the names of each lipid species is shown in Figure S5. A combined analysis of acylcarnitines from males and female is shown in Supplementary Figure S6. Fold changes, p-values, and q-values are provided in Supplementary Table S3.