Fig. 5.

Short-wavelength light-dependent behaviors in PP1-deficient fish and siblings. (A) Schematic representation of the three-dimensional behavior recording system. (B) Spectral distributions of light used in behavior tests. Short-wavelength light (S, magenta) was used as the stimulus, while the standard white LED (dotted curve) was used to keep fish near the surface before recordings. Blue light served as the background light. (C) Fish trajectories during behavior involving retinal photoreception when the light turned off (Top) and on (Bottom). (D and E) Comparison of behavioral traces showing fish z-position changes during short-wavelength light exposure under conditions with (D) or without (E, i.e., dark conditions) blue background light. (F and G) Quantification and comparison of the total ΔZ (Top) and distance (Bottom) in (D and E) during onset or offset of the short-wavelength light between PP1^+/− and PP1^−/− fish (Wilcoxon rank sum test). Experiments were performed using 10 to 11 dpf zebrafish larvae.