Figure 1

Panx1 ablation affects responsiveness to moving gratings

(A) Moving gratings are presented to immobilized 6-day post-fertilization larvae of both genotypes. The eye movements were video recorded and analyzed offline using Stytra (Vilim Stih et al., PLOS Computational Biology, 2019).

(B) Traces of angular horizontal eye movements of both genotypes at 20% and 100% light. n = 3 larvae (C) Positive responses (%) of larvae stimulated by moving gratings at 20% and 100% light. n = 12 larvae.

(D) Time between saccades is identified as the time between the maximum amplitude of a saccade and the onset of a new saccade.

(E) Saccade amplitudes are identified as the maximum angle (in degrees) reached when eyes jerked in response to the stimulus presented at 3.6°/sec.

(F) Saccade duration is calculated as the time between the onset and the peak of the eye movement (ms).

(G, H) Immunohistochemistry-based detection of Panx1a in the brain and retina using the anti-Panx1a antibody from.¹⁷ Arrows in (G) indicate immunoreactive arborization fields in a cross-section of a whole head (6dpf). Arrows in (H) indicate the optic nerve (ON), inner plexiform layer (IPL), retinal ganglion cell layer (RGCL), and lens epithelium (LE) in the retina. The number of animals used for analysis, Panx1a^+/+ (20%) n = 8, Panx1a^+/+ (100%) n = 3, Panx1a^−/− (20%) n = 12 and Panx1a^−/− (100%) n = 5. Statistical analysis: Welch’s t test; p-value significance ∗ <0.05 ∗∗ <0.01 ∗∗∗ <0.005 ∗∗∗∗ <0.001. Scale bars, (G) (150 μm), (H) (50 μm).