Knockout of scamp5a in zebrafish results in Parkinson disease like phenotype. A Measurement of dopamine concentrations in the brains of 5 dpf WT larvae and scamp5a−/− larvae by ELISA. DA, dopamine. WT, wild type. B Measurement of dopamine concentrations in the brains of 9 dpf WT larvae and scamp5a−/− larvae. scamp5a KO resulted in reduced dopamine concentrations in zebrafish. C–E Evaluation of thigmotaxis in 6 dpf WT (n = 13) and scamp5a−/− (n = 18) larvae based on the distance from the well center. C Recorded locomotor trace of 6 dpf larvae. D Quantification of the total distance traveled by 6 dpf larvae. E Quantification of the average velocity of 6 dpf larvae. F–I Analysis of individual larvae performing the light–dark test. WT larvae (n = 26), scamp5a−/− larvae (n = 13), the scamp5a−/ −larvae treated with L-dopa (n = 22) and pramipexole (n = 20) or injected with human SCAMP5 (n = 20). F Recorded locomotor trace of 6 dpf larvae during the light–dark test as indicated. G Quantification of the distance traveled of 6 dpf larvae for one minute at different stages. H Quantification of the total distance traveled by 6 dpf larvae. I Quantification of the average velocity of 6 dpf larvae. The data in panel A, B, D, E, G–I are presented as mean ± SEM. n ≥ 3 independent experiments. Significance levels are denoted as follows: **P < 0.01, ***P < 0.001
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