Fig. 2

tg(gSAIzGFFD2459B) is inserted in the second exon of the rx3 gene. A Screenshot of the zTrap database showing the insertion of the tg(gSAIzGFFD2459B) transgene in the rx3 locus. B Graphic diagram representing the insertion, specifically in the second exon of rx3. Red arrows represent the primers used to validate the insertion. C Gel electrophoresis of PCR products amplified from the genomic DNA of gal4 heterozygous (GFP-positive) embryos shows a 343 bp band (n = 7), whereas no amplification is observed from wild-type embryo genomic DNA. D Chromatogram of the sequencing of the PCR product from the insertion validation. The green section corresponds to a part of the rx3 second exon, the orange section represents the plasmid insertion site of the transgenic line, and the yellow section shows a part of the Tol2 arm, corresponding to the insertion of the transgene. E The expression patterns of the transgene (green, left) and rx3 (purple, right) in 2-somite stage embryos are similar. The embryos shown in panel E are distinct individuals but are at the same developmental stage. Scale bar = 100 µm