Fig. 6

Screening for hox genes responsible for the zebrafish pectoral fin formation. (A) Schematic representation of the genetic screening for hox genes involved in the specification of pectoral fins in zebrafish. (B–G) Dorsal views of live mutant larvae at 3 dpf were obtained during the screening. After capturing images, genotyping, and DNA sequencing in target hoxb genes were conducted. For the mutants illustrated in (C–G), hoxb genes are shown with frameshift mutations introduced. Detailed information is provided in Supplementary file 1A. (H–J) Dorsal views of hoxba−/−;hoxb5b−/− and hoxba−/−;hoxb5b−/−;hoxb6b−/− larvae at 3 dpf. (K–M) Expression patterns of tbx5a in hoxba−/−;hoxb5b−/− and hoxba−/−;hoxb5b−/−;hoxb6b−/− mutants at 30 hpf. Arrowheads indicate the presumptive positions of pectoral fin buds. All images are captured from the dorsal side. Scale bars represent 100 µm.