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Secondary M. abscessus growth in primary granulomas is proportional to M. marinum ESX1-dependent granuloma maturation. A Quantification of M. abscessus colonization of primary M. marinum granulomas at 3 dpsi when infected at 3 or 4 dppi, 3 dppi n = 50, 4 dppi n = 42, two-sided T test. B Representative images of M. abscessus colonization of primary M. marinum granulomas at 3 dpsi treated with isoniazid immediately after secondary infection. C Quantification of primary M. marinum burden in 3 dpsi animals infected with M. abscessus and treated with isoniazid immediately after secondary infection, Control n = 17, Isoniazid n = 24, two-sided T test. D Quantification of M. abscessus colonization of primary M. marinum granulomas at 3 dpsi treated with isoniazid immediately after infection, Control n = 17, Isoniazid n = 24, two-sided T test. E Quantification of animals with at least one M. abscessus colonization event of primary WT and ΔESX1 M. marinum granulomas at 1 dpsi. Data is pooled from two independent experiments. WT n = 27 positive, 3 negative, ΔESX1 n = 24 positive, 13 negative, two-sided Fisher’s Exact Test. F Quantification of macrophage recruitment to WT vs ΔESX1 M. marinum granulomas across 4-22 hpsi. WT n = 10, ΔESX1 n = 12, two-sided T test. G Quantification of M. abscessus colonization of primary WT and ΔESX1 M. marinum granulomas at 3 dpsi. WT n = 17, ΔESX1 n = 22, two-sided T test. Data are presented as mean values +/- SD.
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