Fig. 6

BOM identifies motifs that drive cell type-dependent activity.

a The top 5 ranked SHAP motifs for HepG2 and Gm12878 cells were implanted into a common template sequence (2 copies of the motif in each sequence) to construct synthetic enhancers (SREs) (HepG2 SRE n = 5, Gm12878 SRE n = 5). Each SRE was inserted into a reporter containing a minimal TATA promoter and transfected into both cell types. b Boxplot showing the log2-fold change in the number of SREs accounting for the transfection efficiency and template enhancer activity. Two replicates of the assays were performed. p-values from two-tailed t tests are shown. The quartiles in the box plots represent the 25^th, 50^th (median line), and 75^th percentiles. The interquartile range (IQR) is the difference between the 75^th and 25^th percentiles. The upper and lower whiskers extend to the maximum and minimum values within 1.5 IQR above the 75th percentile and below the 25th percentile, respectively. Source Data are provided as a Source Data file.