Fig. 5

HMH attenuates mortality, abnormalities, and proinflammatory mediator expression in LPS-microinjected zebrafish larvae. (A) Representative image of 3 dpf larvae (n = 10 in each group, triplicated) exposed to 0–20 mg/mL HMH for 48 h. (B) The survival rate of 3 dpf zebrafish larvae with 0–5 mg/mL HMH treatment. Zebrafish larvae at 3 dpf were microinjected with LPS (0.5 mg/mL, 2 nL per larva) and treated with 0–5 mg/mL HMH. (C) The survival rate of zebrafish larvae was determined under a stereomicroscope. (D) Representative images of LPS-microinjected zebrafish larvae. (E) Zebrafish larvae were stained with 10 μM DAF-FMDA for 10 min, and the images were captured using a CELENA S Digital Imaging System. (F) Total RNA was extracted at 18 h, and RT-PCR was performed to evaluate the expression of iNOS and COX-2a. The relative density of iNOS and COX-2a expression was shown relative to β-actin. ∗∗∗p < 0.001 vs. untreated zebrafish larvae and ^###p < 0.001 vs. LPS-microinjected zebrafish larvae.