Figure 5

cGAS-STING pathway control proliferation in telomeric dysfunction.

(A) Representative immunofluorescence images of proliferation and apoptosis. Scale bar = 10 µm. (B) Quantification of proliferation in the skin (n_WT = 5, n_tert-/- = 6, n_sting-/- = 6, n_{tert-/- sting-/-} = 6 WT vs tert-/- p = 0.0001, sting-/- vs tert-/- p = 0.0006, tert-/- vs tert-/- sting-/- p = 0.024). (C) Quantification of proliferation in the testis (n_WT = 6, n_tert-/- = 6, n_sting-/- = 6, n_{tert-/- sting-/-} = 6, WT vs tert-/- p = 0.00007, sting-/- vs tert-/- p = 0.00002, tert-/- vs tert-/- sting-/- p = 0.0006). (D) Quantification of proliferation in the kidney marrow (n_WT = 8, n_tert-/- = 8, n_sting-/- = 6, n_{tert-/- sting-/-} = 8, WT vs tert-/- p = 0.002, sting-/- vs tert-/- p = 0.004). (E) Quantification of proliferation in the intestine (n_WT = 6, n_tert-/- = 5, n_sting-/- = 6, n_{tert-/- sting-/-} = 8). (F) Representative hematoxylin eosin staining of intestine, insets with yellow lines representative of lamina propria thickness. Scale bar = 100 µm. (G) Quantification of lamina propria width (n_WT = 4, n_tert-/- = 4, n_sting-/- = 4, n_{tert-/- sting-/-} = 4, WT vs tert-/- p = 0.042, sting-/- vs tert-/- p = 0.048). (H) RT-qPCR analysis of YAP-TAZ pathway targets in the intestine (n_WT = 4, n_tert-/- = 4, n_sting-/- = 5, n_{tert-/- sting-/-} = 5, ctfg: WT vs tert-/- p = 0.016 sting-/- vs tert-/- p = 0.005, tert-/- vs tert-/- sting-/- p = 0.044; cyr61: WT vs tert-/- p = 0.030, sting-/- vs tert-/- p = 0,023, tert-/- vs tert-/- sting-/- p = 0.046). (I), RT-qPCR analysis of YAP-TAZ pathway targets in the testis (n_WT = 7, n_tert-/- = 8, n_sting-/- = 6–7, _{tert-/- sting-/-} = 7–8, cftg: tert-/- vs tert-/- sting-/- p = 0.008, cyr61: tert-/- vs tert-/- sting-/- p = 0.011). (J) Representative hematoxylin eosin staining of testis. Scale bar = 100 µm. (K) Quantification of mature sperm area (n_WT = 4, n_tert-/- = 4, n_sting-/- = 4, n_{tert-/- sting-/-} = 4, WT vs tert-/- p = 0.0002, sting-/- vs tert-/- p = 0.00007, tert-/- vs tert-/- sting-/- p = 0.006). All data were presented as the mean ± s.e.m.; *p < 0.05; **p < 0.01, ***p < 0.001, using a one-way ANOVA and post hoc Tukey test. Source data are available online for this figure.