Loss of lmx1bb disrupts kidney formation and function. (A) Graphs showing percentage of lmx1bb larvae within each oedema classification at 5 dpf (left) and 7 dpf (right). N=9 for wild type; 16 for lmx1bb−/+; 19 for lmx1bb−/− mutants. (B) Schematic showing normal layout of kidney glomerulus. Electron micrographs images of the glomeruli of (C) wild type, and (D,E) lmx1bb mutants at 6 dpf. For C, pink and white arrowheads indicate interdigitating podocyte foot processes, white dashed lines show GBM, and pink asterisks indicate fenestrations. For D and E, white and purple arrowheads indicate possible interdigitating foot processes, white bracket shows region of foot processes. BV, blood vessel; EC, endothelial cell; F, fenestrations; FP, foot processes; G, glomerulus; GBM, glomerular basement membrane; gbm, partial glomerular basement membrane; IDP, interdigitating podocyte foot processes (indicated by alternating foot processes of different electron densities); M, mitochondria; N, nephron; N(EC), nucleus of endothelial cell; N(P), nucleus of podocyte cell; P, podocytes; RBC, red blood cell; US, urinary space. Scale bars: 1 μm for C; 500 nm for D and E. N=3 for both.
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