Fig. 1
- ID
- ZDB-FIG-250903-31
- Publication
- Ma et al., 2025 - High temperature induces reproductive dysfunction in male zebrafish via DNA methylation-mediated tssk6 downregulation
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High temperature reduced fertility and sperm quality in zebrafish. (A) Schematic diagram of experimental design. The control group was maintained at 28℃ (blue line). HT treatments (red line) included 33℃ short-term (120–141 dph) and 33℃ long-term (1–141 dph). Afterward, the fish recovered at 28℃ for 7 days (141–148 dph). Samples were collected at four time points, marked by triangles. (B) Effects of HT on fertilization rate (2 hpf), mortality rate (24 hpf) and hatching rate (72 hpf) of offspring embryos (n = 360), respectively. Effects of HT on sperm density (C) and sperm motility (D) (n = 24). (E) Effect of HT on ATP contents in sperm (n = 24). *P < 0.05 and **P < 0.01, compared with 28℃ group. ##P < 0.01, compared with 33℃ short-term group. Data were presented as means ± SD. |