Fig 6

Altered size and cell composition of CMZ-derived radial cohorts with loss of Sema3fa.

EdU labelling of retinas of 20 dpf zebrafish larvae incubated for 4 hrs with EdU at 5 dpf. A-D) Examples of radial cohorts of EdU-labelled cells in the central retina of separate WT (A, B) and sema3fa^ca304 (C, D) fish. Panels are separate examples from different fish. E) Number of EdU+ cells (counted in every second section) in each radial cohort averaged per retina (WT, n = 17 larvae; mutant, n = 14 larvae; N = 2 independent replicates). F-H) The averaged distribution of EdU+ cells across the three major layers of the neural retina within individual radial cohorts of each larvae; outer nuclear layer (ONL) (F), inner nuclear layer (INL) (G), and retinal ganglion cell layer (RGCL) (H). A larger percentage of cells of the radial cohorts reside within the RGC layer in the mutant as compared to the WT. I) Layer distribution of cells for each radial cohort of a representative WT and sema3fa^ca304 retina. J-M) Percentages of cells in each retinal lamina were calculated for individual radial cohorts, and these percentages averaged for each retina (WT, n = 17 larvae; mutant, n = 14 larvae). The standard deviations for these averages are graphed in J-M. For all graphs, data points represent individual embryos, error bars are standard deviation. Statistical analyses use Mann Whitney U-test for E-H and a Levene’s test for the equality of variance for J-M. INL, inner nuclear layer; ONL, outer nuclear layer; RGCL, retinal ganglion cell layer. Scale bar is 50 µm for A-D.