Larval locomotion behaviour was similarly affected in mecp2−/− fish bred from homozygous parents (top panel) and heterozygous parents (bottom panel). a Baseline locomotion prior to PTZ-treatment for 5 dpf larvae from homozygous breeding showed that mecp2−/− (n = 117) larvae moved significantly shorter distances than mecp2+/+ (n = 203). b Distances moved by mecp2+/+ (blue; n = 33–35) and mecp2−/− (red; n = 19–20) fish from homozygous parents were similar during baseline (20 min before; lighter colours) and showed similar increases during PTZ-treatment (20 min, darker colours). c Baseline locomotion prior to PTZ-treatment for 5 dpf larvae from heterozygous mecp2+/− crosses showed that mecp2−/− (n = 68) larvae moved significantly shorter distances than mecp2+/+ (n = 90). d The distances moved were similar during baseline (20 min before; lighter colours) and the hyperlocomotion during PTZ-treatment (20 min, darker colours) was also similar in 5 dpf mecp2+/+, mecp2+/−, and mecp2+/+ fish derived from heterozygous breeding. Sample sizes were mecp2+/+ (blue; n = 41–65), mecp2+/− (purple; n = 86–90) and mecp2−/− (red; n = 35–37). Significant effects of PTZ-treatment within genotypes are indicated in grey. ****p < 0.0001 and *p < 0.05. Mean values shown as dotted lines and brackets mark SEM (a and c) and bars correspond to mean ± SEM (b and d)
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