Fig. 5

DRV formulations are quickly internalised into MOLM-13 AML cells. MOLM-13 cells were incubated with rhodamine-labelled fluorescent dehydration-rehydration vesicles (F-DRV), that were either empty or loaded with cyclodextrins (CD), at 2 % v/v concentration for up to 8 h. After incubation, the cells were washed twice in PBS and their red fluorescent intensity analysed using an Accuri C6 flow cytometer (A, B). At least 10,000 events were collected, and the median fluorescence from each sample was determined. See Supplementary Fig. S2 for examples of the gating strategy. The results represent the mean ± SD of either two experiments (F-DRV, F-DRV + SGM) or a single experiment (F-DRV + HP-γ-CD, F-DRV + SBE-β-CD), all performed in triplicates. Results are provided as direct measurements from the flow cytometer (A) or adjusted for the relative fluorescence intensity of each formulation (B). Cells incubated for 2 h were also washed twice in PBS, fixed in 2 % buffered formaldehyde (pH 7.4 PBS), and imaged with an Andor Dragonfly 505 confocal microscope fitted with a CFI SR HP Apo TIRF 100× objective (C-G). Scale bars in the confocal micrographs represent 15 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)