Fig. 3

Illustrative images of the endpoints and sublethal effects observed in zebrafish embryos during four days of exposure with MDMB-4en-PINACA (A), deschloroketamine (DCK) (B), and 2-fluorodeschloroketamine (2F-DCK) (C) at one (1), two (2), three (3), and four (4) days post-fertilization (dpf). The effects were: A2 – lack of heartbeat and degrees of lack of tail detachment; A3 – lack of heartbeat and lack of somite formation; A4 – lack of heartbeat and lack of somite formation; B2 – pericardial edema and lack of pigmentation; B3 – pericardial edema and blood clotting; B4 – scoliosis; C1 – coagulation; C3 – lack of heartbeat and pericardial edema; C4 – pericardial edema and blood clotting; D1, D2, and D4 corresponds to the negative control, with normal development at 24 h, 48 h, and 96 h, respectively.