Fig. 1

wdr5 is required for HSPC survival in the CHT region. (A) IF (Left) and statistical data (Right) showing the expression pattern of Wdr5 protein in Tg(cmyb:EGFP). The yellow boxes (enlarged views shown in the right side) denote Wdr5 signal colocalization in the cmyb+ HSPCs. Error bar, mean ± SD, ****P ≤ 0.0001. (Scale bar, 5 μm). (B) WISH (Left) and statistical data (Right) showing the expression of cmyb in the CHT region of siblings and wdr5 mutants at 2 dpf, 3 dpf, and 4 dpf. The black arrowheads denote cmyb+ HSPCs. Error bar, mean ± SD, ****P < 0.0001. (Scale bar, 100 μm). (C) Confocal imaging (Left) and statistical data (Right) showing cmyb+ HSPCs in the CHT region from siblings and wdr5 mutants with Tg(kdrl:mCherry;cmyb:EGFP) background at 2 dpf and 3 dpf. Error bar, mean ± SD, ****P ≤ 0.0001. (Scale bar, 20 μm). (D) qRT-PCR showing the expression of cmyb in the CHT region of siblings and wdr5 mutants at 2 dpf and 3 dpf. n = 3 replicates. Error bar, mean ± SD, *P = 0.0346, ****P = 0.0001. (E) TUNEL (Left) and statistical data (Right) showing the apoptotic cmyb+ cells (white arrowheads) in the CHT region from siblings and wdr5 mutants with Tg(cmyb:EGFP) background at 2 dpf. Error bar, mean ± SD, ****P ≤ 0.0001. (Scale bar, 50 μm). (F) qRT-PCR showing the expression of baxa, baxb, boxa, boxb, badb, bcl2a, and sival in the CD41+gata1− HSPCs from the CHT region of siblings and wdr5 mutants with Tg(CD41:GFP; gata1:dsRed) background at 2.5 dpf. Error bar, mean ± SD, *P ≤ 0.05, **P ≤ 0.01.