Fig. 1
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Left: optical microscope images of different crystal-containing tissues in adult zebrafish, showing crystals with varying morphologies. Middle: TEM images of biogenic guanine crystals isolated from WT zebrafish operculum, eye and body, showing increasingly more pronounced (012) facets and underexpressed (001) facets. Right: TEM images of in vitro formed (synthetic) crystals mirror the trend observed in the isolated biogenic crystals, with more pronounced (012) facets as hypoxanthine levels increase. We note that the difference in contrast between the synthetic and biogenic crystals is because of the synthetic crystals being larger and thicker than their biogenic counterparts. A schematic illustration of the in vitro and in vivo observations revealing differences in crystal morphology. Crystal isolation was performed on four independent biological replicates. |