Figure 6

Macrophage behavior following tail amputation in cxcr4b crispants. (A) Expression levels of cxcr4b in zebrafish macrophages, as determined via RNA-seq. Bar plots represent the mean ± S.E.M. (B) Relative expression of cxcr4b in macrophages of elf1 crispants compared to the control group at 3 dpf. Unpaired, two-tailed Student’s t-test results for n = 4 are shown as mean ± S.E.M. Statistical significance indicated as **** p < 0.0001. (C) Time-lapse images showing macrophage infiltration in controls and cxcr4b crispants at 3 dpf. Imaging of the caudal fin region was initiated two hours post-amputation. The white dashed line marks the position of transection. Scale bar: 50 μm. (D) Quantification of macrophage numbers within the damage regions shown in (C). Data were analyzed using two-way ANOVA followed by Sidak’s tests, with n ≥ 10, and shown as mean ± S.E.M. Statistical significance indicated as * p < 0.05, ** p < 0.01, and *** p < 0.001.