FIGURE

Figure 2

ID

ZDB-FIG-250315-83

Publication

Muñoz-Garcia et al., 2025 - Interleukin-34 orchestrates bone formation through its binding to bone morphogenic proteins

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Figure 2

Bone mineral and histologic alterations associated with IL34 genetic invalidation in mouse. (A) Comparative analyses of skull bones mineralization levels between Il34^+/+, Il34^-/-, Il34^+/+ injected with IK22.5 antibody, Il34^-/- injected with IK22.5 antibody and Il34^+/+ injected with Sheff.5 antibody mice at age of 15 days, using profile views of the microCT scan 3D reconstructions. The color density ranges from black (lower mineralization) to clear blue (higher mineralization). (B) Comparative analyses of tibias mineralization levels between Il34^+/+, Il34^-/-, Il34^+/+ treated with IK22.5 antibody and Il34^-/- treated with IK22.5 antibody mice at age of 15 days, using longitudinal views of the microCT scan 3D reconstructions. (C) Comparative analysis of the bone volume (BV)/total volume (TV) ratio between Il34^+/+, Il34^-/-, Il34^+/+ treated with IK22.5 antibody and Il34^-/- treated with IK22.5 antibody in bone of different anatomical sites: the mandible, the vertebra, the skull and the tibia. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns: not significant. n=8 except for Il34^-/- + IK22.5 (n=4). (D) Comparative analysis of the bone mineral density (BMD) between Il34^+/+, Il34^-/-, Il34^+/+ treated with IK22.5 antibody and Il34^-/- treated with IK22.5 antibody in bone of different anatomical sites: the mandible, the vertebra, the skull and the tibia. Two areas were considered for the tibia, the trabecular and the cortical. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns: not significant. n=8 except for Il34^-/- + IK22.5 (n=4). (E) Chondrocytes stained by safranin-O staining of tibia longitudinal sections at the level of the proximal epiphysis performed for Il34^-/- and Il34^+/+ mice injected or not with the IK22.5 antibody. (F) Tartrate resistant acid phosphatase (TRAP) and Osterix (Osx/SP7) dual-staining of tibia longitudinal sections at the level of the proximal epiphysis performed for Il34^-/- and Il34^+/+ mice injected or not with the IK22.5 antibody. TRAP red staining for osteoclast cells. OSX brown staining for pre-osteoblasts cells. The scales are given as bars with the corresponding values in the lower part of each histological view.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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