Characterization of cped1 expression. (A) Alternative splicing events for cped1. Top: exon-intron maps for the two cped1 transcript isoforms. Bottom: closeup views of exons 12, 17, and 19 show regions of these exons that vary in usage (denoted by the dotted red lines). The top two rows show how these exon regions differ between the two cped1 transcript isoforms. The remaining rows show these exon regions at various stages of zebrafish development, from 2 cell stage up to 5 dpf, and are colored by usage, eg, red indicates 100% usage in the exon, blue indicates 0% usage in the exon. Gray indicates no transcript evidence was detected at the specified developmental stage. Images for the three exonic regions were obtained from the MeDAS database,40 cropped, and assembled into a single image. (B and C) Bar plots showing average cped1 expression in multiple cell/tissue types. (B) cped1 expression in zebrafish embryos/larvae, from the zebrafish scRNA-seq dataset published in28. The top 45 cell populations with the highest cped1 expression are shown. Osteoblasts are highlighted by an arrow. (C) cped1 expression in adult zebrafish, as reported in the VASTDB database.41 Bone is highlighted by an arrow. (D) RT-PCR for cped1 expression in tissues from wildtype adult fish. Primers amplified a region spanning exons 14 through 16. Amplification of β-actin2 was performed in parallel and served as a loading control. Bone, vertebral bone; Brain: whole brain; dpf, days post fertilization; Int, intestine; Mus, muscle; SB, swim bladder, Test, testes.
|
