Fig 2

SVCV infection enhances hypoxia signaling in zebrafish and zebrafish cells. (A) qPCR analysis of SVCV-N, SVCV-P, SVCV-M, SVCV-G, and SVCV-L mRNA in zebrafish larvae (3 dpf) with or without infection with SVCV (5 × 10⁷ TCID₅₀/mL). (B) qPCR analysis of ldha, glut1, vegfaa, phd3, and pdk1 mRNA in zebrafish larvae (3 dpf) with or without infection with SVCV (5 × 10⁷ TCID₅₀/mL). (C) Heatmap for the selected responsive genes of RIG-I-like receptor signaling pathway in zebrafish larvae (3 dpf) with or without infection with SVCV (5 × 10⁷ TCID₅₀/mL). (D) Heatmap for the selected responsive genes of HIF-mediated hypoxia signaling pathway in zebrafish larvae (3 dpf) with or without infection with SVCV (5 × 10⁷ TCID₅₀/mL). (E) Gene Ontology (GO) enrichment analyses for the DEGs were performed using the cluster Profiler version 3.8. in zebrafish larvae (3 dpf) with or without infection with SVCV (5 × 10⁷ TCID₅₀/mL). (F) qPCR analysis of SVCV-G mRNA in ZFL cells infected with an increasing amount of SVCV. (G) qPCR analysis of ifn1 mRNA in ZFL cells infected with an increasing amount of SVCV. (H, I) qPCR analysis of vegfaa (H) and glut1 (I) mRNA in ZFL cells infected with an increasing amount of SVCV. (J) qPCR analysis of SVCV-G mRNA in ZF4 cells infected with an increasing amount of SVCV. (K) qPCR analysis of ifn1 mRNA in ZF4 cells infected with an increasing amount of SVCV. (L, M) qPCR analysis of vegfaa (L) and glut1 (M) mRNA in ZF4 cells infected with an increasing amount of SVCV. (N) HRE reporter activity in ZF4 cells with or without infection of SVCV.