FIGURE

Figure 2

ID

ZDB-FIG-241219-106

Publication

Abbas et al., 2024 - gnas Knockdown Induces Obesity and AHO Features in Early Zebrafish Larvae

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Figure 2

Western blot analysis of the knockdown efficiency of the three gnas Morpholino (MO) doses at 48 hpf. (a) Representative blot of the Gα_sS expression level in non-injected control (UI Ctrl) and standard control (Std Ctrl) embryos compared to those injected with 1, 3, and 5 ng of gnas MOs. The Std Ctrl embryos were injected with 5 ng of Std Ctrl MO. Whole zebrafish tissue lysates were used to extract total protein (~40 µg loaded per well) and GAPDH served as a loading control. (b) The band intensities were quantified using ImageJ software, followed by normalization of the Gα_sS band intensity to that of GAPDH. The Gα_sS expression level was expressed as a percentage of that in the UI Ctrl embryos. One-way ANOVA followed by Tukey’s multiple comparisons test were performed (**** p < 0.0001). Each bar represents the mean ± SEM (n = 4).

Expression Data

Antibody:	Ab1-gnas
Fish:	AB AB + MO1-gnas + MO2-gnas
Knockdown Reagents:	MO1-gnas MO2-gnas
Anatomical Term:	whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-gnas + MO2-gnas
Knockdown Reagents:	MO1-gnas MO2-gnas
Observed In:	whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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