Figure 1

Larvae exhibit variable locations and sizes of haematoma, and resolution is spontaneous. (A) Representative dorsal view, inverted images of gata1:dsRed fluorescence in a single larva (Fish 7) from 2, 3, 4 and 5 dpf showing haematoma clearance and restoration of circulation. Scale bar = 100 µm. (B) The number of haemorrhages in ICH+ larvae over time (n = 7) **P = 0.0068, F(2.290, 13.74) 2.638 (C) the volume of the haemorrhages **P = 0.0042 *P = 0.0119, F(2.047, 12.28) = 15.23. (D) The surface area of the haemorrhage *P = 0.012, **P = 0.0023, ##P = 0.0024, F(1.934, 11.60) = 21.27 and (E) the surface area to volume ratio of the haemorrhage **P = 0.002, F(1.711, 10.27) = 9.793. Symbol points pair time data for each individual larva. Data were analysed using a repeated measures one-way ANOVA with uncorrected Fishers post hoc analysis to account for no multiple comparisons (F) Representative images of larvae stained for haemoglobin at 2 (n = 24), 3 (n = 28), 4 (n = 45) and 5 (n = 27) dpf shows the distribution of haematoma across the brain changes over time (white arrows). Scale bar = 75 µm. (G) Percentage of larvae with visible haemorrhage over time. Analysis generated from data in (F). (H) Location of bleeds over time as scored from larvae in (F). dpf, days post-fertilisation; ICH, intracerebral haemorrhage; ns, non-significant; Pro, prosencephalon; mes, mesencephalon; rhom, rhombencephalon.