Phylogenetic relationships of the ANKH amino acid sequence in vertebrate organisms. (a) Results from neighbor-joining tree analysis using the out-group taxon (Eptatretus burgeri) demonstrate the relationships between vertebrate organisms. Bootstrap analysis was performed using 10,000 replicates, and these bootstrap values are included. Monophyletic clustering of teleost fish for Ankha and Ankhb are represented by red and blue branches, respectively. (b) The neighbor-joining tree analysis includes human (Homo sapiens), mouse (Mus musculus), and zebrafish (Danio rerio) Ankha and Ankhb, and the out-group taxon (Eptatretus burgeri) includes values from 10,000-replicate bootstrap analysis. (c) Matrix of pairwise amino acid sequence identities for Ankh/Ankha and Ankhb across various vertebrate species determined using percentage identity matrix analysis. Boxes with a black background that have no values represent self-comparisons. High sequence identity is represented by red, and white shades highlight comparisons with lower sequence identity. Squares that have 90 to 100% identity are shown with a dark pink background, sequence identity values from 80–90% are represented in light pink, sequence identity values from 50–80% in white, and sequence identity values below 50% are represented with a white background. Each identity value is rounded to the nearest whole number.
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