Piezo 1, Piezo 2 immunoreactivity in the canal neuromasts of the zebrafish (D. rerio) lateral line system. (a) Piezo 1 immunoreaction in sensory hair sensory cells (arrows), maturing sensory hair cells (blue arrow heads), nerve (gallon arrow), and bone (asterisk) was observed; (b) hair sensory cells (arrows), maturing sensory hair cells (blue arrow heads), nerve (gallon arrow) s100 immunopositive; (c) Piezo 1/s100p double-stained in sensory hair cells (arrows) and maturing sensory hair cells (blue arrow heads) was observed. Moreover, the nerve (gallon arrow) showed a lower double immunoreaction; (d) Piezo 2 immunoreaction in sensory hair sensory cells (arrows), maturing sensory hair cells (blue arrow heads), and bone (asterisk) was observed; (e) sensory hair sensory cells (arrows) and maturing sensory hair cells (blue arrow heads) s100 immunoreactive; (f) Piezo 2/s100 double-stained in sensory hair sensory cells (arrows) and maturing sensory hair cells (blue arrow heads). Magnification 40×.
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