RA influences injury repair and regulates K18a.1 expression in the postnatal neural crest. Adult zebrafish subjected to ocular injury were subsequently recovered in system water containing lidocaine (2.5 mg/L) or lidocaine in combination with either 0.1% DMSO, 10 mM DEAB, or 100 nM ATRA. (A–F) Fluorescein tracing shows that corneal healing was remarkably disrupted by treatment with DEAB (E) and ATRA (F). The dashed and solid circles highlight the iris and pupil, respectively, in the zebrafish eye. (G–L) Cryosection (10 μm), followed by immunohistochemical analysis revealed significant stromal edema (K) and epithelial (L) edema in response to alterations in RA levels during ocular wound healing. CEpi, corneal epithelium; CS, corneal stroma; CEndo, corneal endothelium. (M,N) Quantitative analysis of K8 and K18a.1 expression revealed decreased Krt18a.1 expression in response to pharmacological insult during corneal wound healing. *, p-value < 0.05; **, p-value < 0.01; ***, p-value < 0.001; n.s., not significant.
|
