Figure 3

Developmental alterations (A,C) and malformations (B,D) were observed in zebrafish larvae exposed at 96 h post-fertilization to different concentrations of anise (A,B) and lemon (C,D) myrtle extract. The alterations observed were pigmentation delay (Pig. D), developmental delay (Dev. D), yolk sac absorption delay (Yolk Sac AD), yolk sac edema (Yolk Sac E), and cardiac edema (Cardiac E). The malformations observed were localized in the head, eyes, spine, and tail. None of the alternations observed in anise myrtle were significantly different from the control 0 mg/L group. In contrast, in Lemon myrtle, there were substantial alterations and malformations at 240 mg/L. The 480 mg/L group only includes very few survivors. Bar colours represent the concentrations tested in mg/L. Data represent the mean ± SD, n = 60. Asterisks indicate statistical significance when comparing the exposed groups with the control groups: p < 0.05 (*) and p < 0.001 (***).