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Fig. 3

ID

ZDB-FIG-240628-38

Publication

Stamellou et al., 2023 - Inhibition of the glucocorticoid receptor attenuates proteinuric kidney diseases in multiple species

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Fig. 3

Pharmacological inhibition in PAN model. (A) Scheme of the PAN model. Proteinuria in rats was induced by a single intravenous (i.v.) puromycin aminonucleoside injection at 50 mg/kg body weight. The rats were then treated for 9 days with either methylprednisolone or mifepristone by i.p. injection (n = 6–12) (B) UPCR in mg/g creatinine on Day 11 in PAN, healthy control rats and treated rats. (C) PAS staining in methylprednisolone-treated, mifepristone-treated and untreated PAN rats showing no evidence of FSGS. (D) Histological quantification of glomerular desmin expression. (E) Quantification of slit diaphragm density width in PAN rats treated or not with mifepristone. Mifepristone treatment resulted in increased slit diaphragm density compared with vehicle treated rats. Data are expressed as means ± SD. ****P < .0001 by Student's t-test. (G) Images of 3D-reconstructed SIM volumes showing the spatial aspect of the slit diaphragm on the capillary loops in the different groups. The same colors indicate the same Z-position within the total Z-volume of 4.5 µm. Data represent means ± SD *P < .05, **P < .001 by Student's t-test or by 1-way ANOVA followed by Bonferroni's post hoc test.

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