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Fig. 1

ID

ZDB-FIG-240607-94

Publication

Wang et al., 2024 - Improvement in Tol2 transposon for efficient large-cargo capacity transgene applications in cultured cells and zebrafish ( Danio rerio)

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Fig. 1

Design of improved Tol2 transposases with enhanced expression and nuclear targeting A: Schematic of modified Tol2 transposase design. Tol2 transposase is driven by the CMV promoter and engineered for enhanced expression and nuclear targeting by incorporating the QBI SP163 translational enhancer and H2B. B: Fluorescence microscopy images of H1299 cells transfected with EGFP-fused Tol2 transposase constructs: T (wild-type Tol2), ST (SP163-Tol2), and SHT (SP163-H2B-Tol2). Cells were fixed and stained with DAPI to visualize nuclei. Scale bars, 20 μm. C, D: Quantification of normalized EGFP fluorescence intensity in total cell cytosol (C) and nuclei (D). Data are mean±SEM. n=3 independent experiments. *: P<0.05; **: P<0.01; ***: P<0.001; ****: P<0.0001. Student’s unpaired t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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