FIGURE

Fig. 1

ID
ZDB-FIG-240603-1
Publication
Mathavarajah et al., 2023 - Suppressors of cGAS-STING are downregulated during fin-limb regeneration and aging in aquatic vertebrates
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Fig. 1

Early-stage gar regenerative blastema transition into a pro-inflammatory state with elevated cGAS activity. (a) Spotted gar caudal fins were amputated and regeneration was observed over a 32-day period, with regenerative blastema being collected at the indicated timepoints for molecular analysis. Scale bar indicates 1 cm. (b) Interferons and Interleukins are upregulated in blastema at 7 dpa before returning to baseline levels (n = 3). Gene expression of ifnb, ifnc1, il6 (Interleukin 6), and il8 (Interleukin 8-like) was assessed and significantly different from the original amputated fin (AF) only a 7 dpa. (c) 2′3′- cGAMP levels are elevated specifically at 7 dpa in regenerative blastema. 2′3′-cGAMP concentrations were determined from the same caudal fins used for RNA isolation. (d) Gene expression analysis of cGAS-STING pathway orthologs in gar indicate that pml is downregulated to facilitate immune signaling via the cGAS-STING axis. Variation between groups was assessed with a one-way ANOVA, with Tukey′s post hoc analysis for pairwise comparison between groups. ****p < 0.0001.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Exp. Zool. B Mol. Dev. Evol.