Fig.5
- ID
- ZDB-FIG-240503-50
- Publication
- Sun et al., 2012 - A benchmarked protein microarray-based platform for the identification of novel low-affinity extracellular protein interactions
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Quantitative benchmarking of the microarray AVEXIS using interactions within the zebrafish Jam family. (A) All six members of the zebrafish Jam family were expressed as biotinylated baits, purified, normalized, serially diluted, and arrayed on streptavidin-coated slides before detection with an anti-Cd4 primary antibody and a Cy3-conjugated goat anti-mouse secondary antibody. The image shows that similar levels of Jam bait proteins were immobilized on the slide. (B) Graphical representation of expected and observed interactions of different affinities within the zebrafish Jam family. The expected (left panel) interactions within the zebrafish Jam family were grouped according to their affinities (red = high affinity [ |