FIGURE

Figure 1

ID

ZDB-FIG-240426-60

Publication

Fang et al., 2024 - SUMOylation of zebrafish transcription factor Zbtb21 affects its transcription activity

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Figure 1

Zbtb21 is identified as a SUMOylated substrate.

(A) Western blot analyses (anti-HA) of HA-tagged wild type (WT), Zbtb21^K419R, Zbtb21^K419R, Zbtb21^K845R, and Zbtb21^K419/845R mutant proteins expressed in HEK293T cells in the absence or presence with the SUMO conjugating enzyme UBC9 and SUMO1. TUBULIN served as internal control. (B) HA-tagged wild type (WT), Zbtb21^K419R, Zbtb21^K419R, Zbtb21^K845R, and Zbtb21^K419/845R mutant proteins were immunoprecipitated with an anti-HA antibody from HEK293T cells co-expressing SUMO1, and SUMOylated Zbtb21 protein was detected by western blot with an anti-SUMO1 antibody.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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