Fig. 5

Effects of cytokine inhibitors on neuromast regeneration. We cut the tails of Et(HG7L) larvae at 3 days post-fertilization and then cultured them in the absence (Unt) or presence of (a) 0–10 μM LMT-28 (n = 58–60 for each treatment group at 0 dpa), (b) 0–100 μM pentoxifylline (PTX) (n = 40–42 for each treatment group at 0 dpa), (c) 0–25 μM SB431542 (n = 31–39 for each treatment group), and (d) 0–10 μM SB505124 (n = 33–41 for each treatment group at 0 dpa). (e) Larvae from the cross of Et(HG7L) and Tg(−8.0cldnb:NTR-hkiKGR) were treated with 2 mM metronidazole (Mtz) 3 days post-fertilization for 12 h to chemical-genetically ablate neuromasts, washed, and treated without (Unt) or with 25 μM SB431542. We examined the regeneration of neuromast. The % of larvae in each neuromast regeneration phase is presented as described in Figure 1, except that Phase II-1 and Phase II-2 are combined as Phase II (panel on the left). We counted the number of larvae at each phase at designated time points (presented as days post-amputation [dpa]). The percentage of larvae in each phase is shown. n.s. not significant; *p < .05; **p < .01; ***p < .001.