FIGURE

FIGURE 1

ID

ZDB-FIG-240323-27

Publication

Wang et al., 2024 - Deficiency of heme oxygenase 1a causes detrimental effects on cardiac function

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 1

Deletion of hmox1a increases cardiac output in larvae. (A) Scheme of CRISPR/Cas9‐generated 52‐base pair deletion in exon 3 of hmox1a. (B) RT‐qPCR analysis of hmox1a, hmox1b, hmox2a and hmox2b in wild type (WT), heterozygous (HET) and homozygous (KO) hmox1a mutant at 5–6 dpf. The results are presented as fold change compared to WT. RNA was extracted from 5 to 10 pooled larvae. The number of RNA samples used for RT‐qPCR analyses as following: WT n = 5; HET(52del) n = 6; KO(52del) n = 6. (C) Representative images of hmox1a WT, HET(52del) and KO(52del) larvae at 5 dpf showing no obvious gross phenotype. Scale bar: 500 μm. (D–H) Analysis of cardiac function at 5–6 dpf larvae indicating an increase in cardiac output (D) and heart rate (E), and no significant change in stroke volume (F), ejection fraction (G) and end‐diastolic ventricular area normalized to body length (H) in hmox1a KO(52del). WT n = 28; HET(52del) n = 43; KO(52del) n = 30. Data are presented as mean ± SD. One‐way ANOVA with Tukey adjustment for multiple comparisons. *p < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell. Mol. Med.