Fig 4

escapist p404 larvae exhibit increased acoustic startle sensitivity and decreased visual responses at 6 days post fertilization.

(A) Acoustic startle short term habituation assay: i) Average probability of startle for siblings (n = 29 larvae, black) and p404 homozygous mutant larvae (n = 23 larvae, red) in escapist line at baseline (10 acoustic stimuli with 40 sec interstimulus interval), as well as during stimuli that induce habituation (acoustic stimulus presented with 1sec interstimulus interval). Average probability of startle is grouped by stimulus number 1–10, 11–20, 21–30. Any larvae that had an average probability of startle of less than 0.6 during baseline stimuli were omitted from habituation analysis. ii) Habituation index calculated for both siblings and p404 homozygous mutant larvae. Habituation index is calculated using the following formula: % Habituation = [1- ((%Startle Stimulus 21–30)/ (%Startle Baseline))] *100. (B) Dark flash response for sibling larvae (n = 95 larvae) and p404 homozygous mutant larvae (n = 34 larvae). i) O-bend probability, ii) average distance traveled and iii) average distance traveled are shown. For statistical purposes, values were normalized to sibling responses, and student’s t-test was performed to compare mutants and sibling groups with Bonferroni used for multiple comparisons of 86 parameters analyzed during behavioral testing (See S2 Fig for all parameters tested). P-values that are significant after analysis and statistical correction are indicated with asterisks (***).