FIGURE

Fig. 2

ID

ZDB-FIG-240315-29

Publication

Walter et al., 2024 - Functionally-instructed modifiers of response to ATR inhibition in experimental glioma

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Fig. 2

CRISPR-Cas9 genome-wide knockout and activation screens identify novel combination partners for ATRi. a, b Upper half, CRISPR-Cas9 screen analyses using a knockout (Brunello) library in LN229 (a) and LNZ308 cells (b) using 750 nM of AZD6738. Left, 9-square plot of MAGeCK MLE results comparing Brunello sgRNA distributions from DMSO or AZD6738-treated cells to the plasmid library pool. Right, rankview plot illustrating MAGeCK MLE results comparing Brunello sgRNA distributions from AZD6738-treated cells to the corresponding DMSO control. c, d Lower half, CRISPR-Cas9 screen analyses using an activation (Calabrese) library in LN229 (c) or LNZ308 cells (d), respectively, using 1.5 µM AZD6738 for LN229 and 1.4 µM AZD6738 for LNZ308 cells. Left, 9-square plot of MAGeCK MLE results comparing Calabrese sgRNA distributions from DMSO or AZD6738-treated cells to the plasmid library pool. Right, rankview plot illustrating MAGeCK MLE results comparing Calabrese sgRNA distributions from AZD6738-treated cells to the corresponding DMSO control. The respective experimental set-up was used to prioritize genetic vulnerabilities (Brunello library) and resistance mechanisms (Calabrese library) upon ATR inhibition

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Exp. Clin. Cancer Res.