FIGURE

Fig. 2

ID

ZDB-FIG-240315-13

Publication

Abbott et al., 2023 - A novel ERβ high throughput microscopy platform for testing endocrine disrupting chemicals

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

ERβ elicits a strong and reproducible transcriptional response from the PRL array (A) Representative images of dsRED2 smFISH after DMSO, E2 (10 nM), or 4HT (100 nM) treatment for 2-h; (B) quantitation of dsRED2 intensity at the PRL array after a time course treatment with E2 (10 nM) or 4HT (100 nM); C) E2 and 4HT dose-response analysis of the dsRED2 response at 2-h; and D) effect of the indicated antagonists on basal and E2 (10 nM)-stimulated dsRED2 transcription. Cells were treated with 4HT (1 μM), ICI182,780 (ICI, 1 μM), PHTTP (1 μM), actinomycin D (ActD, 1 μg/ml), and flavopiridol (Flavo, 100 nM) ± E2 10 nM *p < 0.05 as compared to DMSO control; **p < 0.05 as compared to E2 treated cells. All experiments are represented as mean and standard deviation from eight technical replicates.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Heliyon